Integrin-linked kinase affects signaling pathways and migration in thyroid cancer cells and is a potential therapeutic target.

ABSTRACT:

Background

Integrin-linked kinase (ILK) is a serine-threonine kinase that regulates interactions between the cell and the extracellular matrix. In many cancers, overexpression of ILK leads to increased cell proliferation, motility, and invasion. We hypothesized that ILK functions as a regulator of viability and migration in thyroid cancer cells.

Methods

Eleven human thyroid cancer cell lines were screened for ILK protein expression. The cell lines with the greatest expression were treated with either ILK small interfering RNA (siRNA) or a novel ILK inhibitor, T315, and the effects were evaluated via Western blot and migration assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assays were performed to assess cell viability.

Results

siRNA against IL

SUBMITTER: Shirley LA 

PROVIDER: S-EPMC4961251 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature