Rapid formation of size-controllable multicellular spheroids via 3D acoustic tweezers.

ABSTRACT: The multicellular spheroid is an important 3D cell culture model for drug screening, tissue engineering, and fundamental biological research. Although several spheroid formation methods have been reported, the field still lacks high-throughput and simple fabrication methods to accelerate its adoption in drug development industry. Surface acoustic wave (SAW) based cell manipulation methods, which are known to be non-invasive, flexible, and high-throughput, have not been successfully developed for fabricating 3D cell assemblies or spheroids, due to the limited understanding on SAW-based vertical levitation. In this work, we demonstrated the capability of fabricating multicellular spheroids in the 3D acoustic tweezers platform. Our method used drag force from microstreaming to levitate cells

SUBMITTER: Chen K 

PROVIDER: S-EPMC4961479 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature