Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module.

ABSTRACT: Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and t

SUBMITTER: Yim N 

PROVIDER: S-EPMC4961865 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature