Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System.

ABSTRACT:

Purpose

Recombinant human endostatin (rhEs) is an angiogenesis inhibitor which is used as a specific drug in the treatment of non-small-cell lung cancer. In the current research, we developed an efficient method for expressing soluble form of the rhEs protein in the periplasmic space of Escherichia coli via fusing with pelB signal peptide.

Methods

The human endostatin (hEs) gene was amplified using synthetic (hEs) gene as a template; then, cloned and expressed under T7 lac promoter. IPTG was used as an inducer for rhEs expression. Next, the osmotic shock was used to extraction of protein from the periplasmic space. The presence of rhEs in the periplasmic space was approved by SDS-PAGE and Western blotting.

Results

The results show the applicability of pelB fusion prot

SUBMITTER: Mohajeri A 

PROVIDER: S-EPMC4961976 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature