Design of DNA-conjugated polypeptide-based capture probes for the anchoring of proteins to DNA matrices.

ABSTRACT: A new method for protein surface functionalization was developed that utilizes DNA-conjugated artificial polypeptides to capture recombinant target proteins from the solution phase and direct their deposition onto DNA-functionalized matrices. Protein capture is accomplished through the coiled-coil association of an engineered pair of heterodimeric leucine zippers. Incorporating half of the zipper complex directly into the polypeptides and labeling these polymers with ssDNA enables the polypeptide conjugates to form intermediate linkages that connect the target proteins securely to DNA-functionalized supports. This synthetic route provides an important alternative to conventional DNA-conjugation techniques by allowing proteins to be outfitted site-specifically with ssDNA while minimizing th

SUBMITTER: Schweller RM 

PROVIDER: S-EPMC4962553 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature