Purification of Plasmodium Sporozoites Enhances Parasite-Specific CD8+ T Cell Responses.

ABSTRACT: Malaria infection caused by Plasmodium parasites continues to cause enormous morbidity and mortality in areas where it is endemic, and there is no licensed vaccine capable of inducing sterile protection. Hyperimmunization with attenuated whole sporozoites can induce sterile protective immune responses targeting preerythrocytic antigens. Most animal models of hyperimmunization rely on sporozoites dissected from mosquito salivary glands and injected without further purification. In BALB/c mice, repeated small doses of P. yoelii sporozoites progressively expand the population of sporozoite-specific CD8(+) T cells. In this study, large secondary doses of unpurified sporozoites unexpectedly led to contraction of sporozoite-specific CD8(+) T cell responses in sporozoite-primed mice. While sporoz

SUBMITTER: Billman ZP 

PROVIDER: S-EPMC4962643 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature