High-temperature LC-MS/MS of permethylated glycans derived from glycoproteins.

ABSTRACT: Various glycomic analysis methods have been developed due to the essential roles of glycans in biological processes as well as the potential application of glycomics in biomarker discovery in many diseases. Permethylation is currently considered to be one of the most common derivatization methods in MS-based glycomic analysis. Permethylation not only improves ionization efficiency and stability of sialylated glycans in positive mode but also allows for enhanced separation performance on reversed-phase liquid chromatography (RPLC). Recently, RPLC-MS analysis of permethylated glycans exhibited excellent performance in sensitivity and reproducibility and became a widely-applied comprehensive strategy in glycomics. However, separating permethylated glycans by RPLC always suffers from peak broa

SUBMITTER: Zhou S 

PROVIDER: S-EPMC4962687 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature