Coupling between alternative polyadenylation and alternative splicing is limited to terminal introns.

ABSTRACT: Alternative polyadenylation has been implicated as an important regulator of gene expression. In some cases, alternative polyadenylation is known to couple with alternative splicing to influence last intron removal. However, it is unknown whether alternative polyadenylation events influence alternative splicing decisions at upstream exons. Knockdown of the polyadenylation factors CFIm25 or CstF64 in HeLa cells was used as an approach in identifying alternative polyadenylation and alternative splicing events on a genome-wide scale. Although hundreds of alternative splicing events were found to be differentially spliced in the knockdown of CstF64, genes associated with alternative polyadenylation did not exhibit an increased incidence of alternative splicing. These results demonstrate that t

SUBMITTER: Movassat M 

PROVIDER: S-EPMC4962795 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature