Critical Role of Glucose Metabolism in Rheumatoid Arthritis Fibroblast-like Synoviocytes.

ABSTRACT:

Objective

Up-regulation of glucose metabolism has been implicated not only in tumor cell growth but also in immune cells upon activation. However, little is known about the metabolite profile in rheumatoid arthritis (RA), particularly in fibroblast-like synoviocytes (FLS). This study was undertaken to evaluate whether changes in glucose metabolism in RA FLS could play a role in inflammation and joint damage.

Methods

Synovium and FLS were obtained from patients with RA and patients with osteoarthritis (OA). The rate of glycolysis after stimulation of FLS with lipopolysaccharide and platelet-derived growth factor BB was measured using glycolysis stress test technology. FLS function was evaluated using a glycolysis inhibitor, 2-deoxy-d-glucose (2-DG). After stimulation of the F

SUBMITTER: Garcia-Carbonell R 

PROVIDER: S-EPMC4963240 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature