Increased DNA Methylation and Reduced Expression of Transcription Factors in Human Osteoarthritis Cartilage.

ABSTRACT:

Objective

To analyze the methylome of normal and osteoarthritic (OA) knee articular cartilage and to determine the role of DNA methylation in the regulation of gene expression in vitro.

Methods

DNA was isolated from human normal (n = 11) and OA (n = 12) knee articular cartilage and analyzed using the Infinium HumanMethylation450 BeadChip array. To integrate methylation and transcription, RNA sequencing was performed on normal and OA cartilage and validated by quantitative polymerase chain reaction. Functional validation was performed in the human TC28 cell line and primary chondrocytes that were treated with the DNA methylation inhibitor 5-aza-2'-deoxycytidine (5-aza-dC).

Results

DNA methylation profiling revealed 929 differentially methylated sites between normal and

SUBMITTER: Alvarez-Garcia O 

PROVIDER: S-EPMC4963260 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature