Conformational IgE epitopes of peanut allergens Ara h 2 and Ara h 6.

ABSTRACT:

Background

Cross-linking of IgE antibody by specific epitopes on the surface of mast cells is a prerequisite for triggering symptoms of peanut allergy. IgE epitopes are frequently categorized as linear or conformational epitopes. Although linear IgE-binding epitopes of peanut allergens have been defined, little is known about conformational IgE-binding epitopes.

Objective

To identify clinically relevant conformational IgE epitopes of the two most important peanut allergens, Ara h 2 and Ara h 6, using phage peptide library.

Methods

A phage 12mer peptide library was screened with allergen-specific IgE from 4 peanut-allergic patients. Binding of the mimotopes to IgE from a total of 29 peanut-allergic subjects was measured by ELISA. The mimotope sequences were mapped on t

SUBMITTER: Chen X 

PROVIDER: S-EPMC4963300 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature