Assessment of whole blood thrombosis in a microfluidic device lined by fixed human endothelium.

ABSTRACT: The vascular endothelium and shear stress are critical determinants of physiological hemostasis and platelet function in vivo, yet current diagnostic and monitoring devices do not fully incorporate endothelial function under flow in their assessment and, therefore, they can be unreliable and inaccurate. It is challenging to include the endothelium in assays for clinical laboratories or point-of-care settings because living cell cultures are not sufficiently robust. Here, we describe a microfluidic device that is lined by a human endothelium that is chemically fixed, but still retains its ability to modulate hemostasis under continuous flow in vitro even after few days of storage. This device lined with a fixed endothelium supports formation of platelet-rich thrombi in the presence of physi

SUBMITTER: Jain A 

PROVIDER: S-EPMC4963439 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature