Early Selection for Smut Resistance in Sugarcane Using Pathogen Proliferation and Changes in Physiological and Biochemical Indices.

ABSTRACT: Sugarcane smut disease, caused by Sporisorium scitamineum, significantly decreases yield and use of resistant cultivars is the most cost-effective measure for disease control. Current field testing methods for identification of smut resistance are time-consuming and hindered by environmental variability. Our goal was to develop an efficient and reliable resistance identification technique that is rapid, performed in a controlled environment, and stable. Nine sugarcane cultivars with different phenotypic resistance levels were selected. TaqMan quantitative real-time polymerase chain reaction analysis was performed to measure copy number changes of smut pathogen in sugarcane buds at 0-7 days after needle puncture inoculation. There was a positive correlation between time after inoculation an

SUBMITTER: Su Y 

PROVIDER: S-EPMC4963460 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature