A Computationally Designed Serological Assay for Porcine Epidemic Diarrhea Virus.

ABSTRACT: The periodic emergence of new infectious agents and the genetic and antigenic evolution of existing agents necessitate the improvement of technology for the rapid development of diagnostic assays. The porcine epidemic diarrhea virus (PEDV) emerged in the United States in 2013, causing severe economic damage to the pork industry. The primary goal of this study was to develop methods to reduce the lead time for serological assay development. An approach involving the computational prediction of diagnostic targets, followed by a rapid synthesis of antigens, was adopted to achieve this objective. To avoid cross-reactivity with other closely related swine coronaviruses, the N protein sequences of PEDV were analyzed to identify sequences unique to PEDV. The potential antigenicity of the identifi

SUBMITTER: Song Y 

PROVIDER: S-EPMC4963506 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature