Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation.

ABSTRACT: Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study, we have used emerging rules of managing adhesion/cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate differentiation, hence better informing biological mechanisms of stem cell growth. Too

SUBMITTER: Roberts JN 

PROVIDER: S-EPMC4963921 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature