ITRAQ-based proteomic profiling reveals different protein expression between normal skin and hypertrophic scar tissue.

ABSTRACT:

Background

A hypertrophic scar is a unique fibrotic disease that only exists in humans. Despite advances in burn care and rehabilitation, as well as progress in the management during these decades, the hypertrophic scar remains hard to cure following surgical methods and drugs for treatment. In this study, we are looking forward to finding the multitude of possible traumatic mechanisms and the underlying molecular signal ways in the formation of the hypertrophic scar.

Methods

We used isobaric tags for relative and absolute quantitation (iTRAQ) labeling technology, followed by high-throughput 2D LC-MS/MS, to determine relative quantitative differential proteins between the hypertrophic scar and normal skin tissue.

Results

A total of 3166 proteins were identified with a

SUBMITTER: Tan J 

PROVIDER: S-EPMC4964291 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature