De Novo Assembly and Characterization of the Transcriptome of Grasshopper Shirakiacris shirakii.

ABSTRACT:

Background

The grasshopper Shirakiacris shirakii is an important agricultural pest and feeds mainly on gramineous plants, thereby causing economic damage to a wide range of crops. However, genomic information on this species is extremely limited thus far, and transcriptome data relevant to insecticide resistance and pest control are also not available.

Methods

The transcriptome of S. shirakii was sequenced using the Illumina HiSeq platform, and we de novo assembled the transcriptome.

Results

Its sequencing produced a total of 105,408,878 clean reads, and the de novo assembly revealed 74,657 unigenes with an average length of 680 bp and N50 of 1057 bp. A total of 28,173 unigenes were annotated for the NCBI non-redundant protein sequences (Nr), NCBI non-redundant nucleo

SUBMITTER: Qiu Z 

PROVIDER: S-EPMC4964485 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature