LC-MS/MS of permethylated N-glycans derived from model and human blood serum glycoproteins.

ABSTRACT: LC-MS/MS is one of the most powerful tools for N-glycan structure elucidation; however, it is still challenging to identify some glycan structures with low abundance. In this study, we investigated the chromatographic behavior of permethylated N-glycans. The relationship between retention times versus molecular weight of dextran, dextrin, and model glycans was investigated. Also, the nonpolar surface area of glycans was calculated and compared to their experimental retention times. Both retention time and nonpolar surface area trends are similar when the intermolecular interaction is included in the calculation. Moreover, retention time corresponds to glycan types and branch types. The N-glycans analysis model, which combines high mass accuracy and retention time, was applied to confirm se

SUBMITTER: Hu Y 

PROVIDER: S-EPMC4964794 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature