Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.

ABSTRACT: The Flaviviridae are a family of viruses that cause severe human diseases. For example, dengue virus (DENV) is a rapidly emerging pathogen causing an estimated 100 million symptomatic infections annually worldwide. No approved antivirals are available to date and clinical trials with a tetravalent dengue vaccine showed disappointingly low protection rates. Hepatitis C virus (HCV) also remains a major medical problem, with 160 million chronically infected patients worldwide and only expensive treatments available. Despite distinct differences in their pathogenesis and modes of transmission, the two viruses share common replication strategies. A detailed understanding of the host functions that determine viral infection is lacking. Here we use a pooled CRISPR genetic screening strategy to co

SUBMITTER: Marceau CD 

PROVIDER: S-EPMC4964798 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature