DNA-Dye-Conjugates: Conformations and Spectra of Fluorescence Probes.

ABSTRACT: Extensive molecular-dynamics (MD) simulations have been used to investigate DNA-dye and DNA-photosensitizer conjugates, which act as reactants in templated reactions leading to the generation of fluorescent products in the presence of specific desoxyribonucleic acid sequences (targets). Such reactions are potentially suitable for detecting target nucleic acids in live cells by fluorescence microscopy or flow cytometry. The simulations show how the attached dyes/photosensitizers influence DNA structure and reveal the relative orientations of the chromophores with respect to each other. Our results will help to optimize the reactants for the templated reactions, especially length and structure of the spacers used to link reporter dyes or photosensitizers to the oligonucleotides responsible f

SUBMITTER: Beierlein FR 

PROVIDER: S-EPMC4965132 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature