Vitamin D Deficiency Accelerates Coronary Artery Disease Progression in Swine.

ABSTRACT:

Objective

The role of vitamin D deficiency in coronary artery disease (CAD) progression is uncertain. Chronic inflammation in epicardial adipose tissue (EAT) has been implicated in the pathogenesis of CAD. However, the molecular mechanism underlying vitamin D deficiency-enhanced inflammation in the EAT of diseased coronary arteries remains unknown. We examined a mechanistic link between 1,25-dihydroxyvitamin D-mediated suppression of nuclear factor-κB (NF-κB) transporter, karyopherin α4 (KPNA4) expression and NF-κB activation in preadipocytes. Furthermore, we determined whether vitamin D deficiency accelerates CAD progression by increasing KPNA4 and nuclear NF-κB levels in EAT.

Approach and results

Nuclear protein levels were detected by immunofluorescence and Western blot.

SUBMITTER: Chen S 

PROVIDER: S-EPMC4965317 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature