Early Molecular Events in Murine Gastric Epithelial Cells Mediated by Helicobacter pylori CagA.

ABSTRACT:

Background

Murine models of Helicobacter pylori infection are used to study host-pathogen interactions, but lack of severe gastritis in this model has limited its usefulness in studying pathogenesis. We compared the murine gastric epithelial cell line GSM06 to the human gastric epithelial AGS cell line to determine whether similar events occur when cultured with H. pylori.

Materials and methods

The lysates of cells infected with H. pylori isolates or an isogenic cagA-deficient mutant were assessed for translocation and phosphorylation of CagA and for activation of stress pathway kinases by immunoblot.

Results

Phosphorylated CagA was detected in both cell lines within 60 minutes. Phospho-ERK 1/2 was present within several minutes and distinctly present in GSM06 cells a

SUBMITTER: Banerjee A 

PROVIDER: S-EPMC4965344 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature