Dataset Information

A Japanese familial case of hypochondroplasia with a novel mutation in FGFR3.

ABSTRACT:

SUBMITTER: Nagahara K

PROVIDER: S-EPMC4965510 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A Japanese familial case of hypochondroplasia with a novel mutation in FGFR3.

Nagahara Keiko K Harada Yuki Y Futami Tohru T Takagi Masaki M Nishimura Gen G Hasegawa Yukihiro Y

Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology 20160720 3

PMID: 27507911

Similar Datasets

Project description:RationaleHypochondroplasia (HCH) is the mildest form of chondrodysplasia characterized by disproportionate short stature, short extremities, and variable lumbar lordosis. It is caused by mutations in fibroblast growth factor receptor 3 (FGFR3) gene. Up to date, at least thirty mutations of FGFR3 gene have been found to be related to HCH. However, mutational screening of the FGFR3 gene is still far from completeness. Identification of more mutations is particularly important in diagnosis of HCH and will gain more insights into the molecular basis for the pathogenesis of HCH.Patient concernsA large Chinese family consisting of 53 affected individuals with HCH phenotypes was examined.DiagnosesA novel missense mutation, c.1052C>T, in FGFR3 gene was identified in a large Chinese family with HCH. On the basis of this finding and clinical manifestations, the final diagnosis of HCH was made.InterventionsNext-generation sequencing (NGS) of DNA samples was performed to detect the mutation in the chondrodysplasia-related genes on the proband and her parents, which was confirmed by Sanger sequencing in the proband and most of other living affected family members.OutcomesA novel missense mutation, c.1052C>T, in the extracellular, ligand-binding domain of FGFR3 was identified in a large Chinese family with HCH. This heterozygous mutation results in substitution of serine for phenylalanine at amino acid 351 (p.S351F) and co-segregates with the phenotype in this family. Molecular docking analysis reveals that this unique FGFR3 mutation results in an enhancement of ligand-binding affinity between FGFR3 and its main ligand, fibroblast growth factor 9.LessonsThis novel mutation is the first mutation displaying an increase in ligand-binding affinity, therefore it may serve as a model to investigate ligand-dependent activity of FGF-FGFR complex. Our data also expanded the mutation spectrum of FGFR3 gene and facilitated clinic diagnosis and genetic counseling for this family with HCH.

Dataset Information

A Japanese familial case of hypochondroplasia with a novel mutation in FGFR3.

Publications

A Japanese familial case of hypochondroplasia with a novel mutation in FGFR3.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets