Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.

ABSTRACT: Doublecortin is a microtubule-associated protein produced during neurogenesis. The protein stabilizes microtubules and stimulates their polymerization, which allows migration of immature neurons to their designated location in the brain. Mutations in the gene that impair doublecortin function and cause severe brain formation disorders are located on a tandem repeat of two doublecortin domains. The molecular mechanism of action of doublecortin is only incompletely understood. Anti-doublecortin antibodies, such as the rabbit polyclonal Abcam 18732, are widely used as neurogenesis markers. Here, we report the generation and characterization of antibodies that bind to single doublecortin domains. The antibodies were used as tools to obtain structures of both domains. Four independent crystal s

SUBMITTER: Burger D 

PROVIDER: S-EPMC4965577 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature