Assembly and Immunological Processing of Polyelectrolyte Multilayers Composed of Antigens and Adjuvants.

ABSTRACT: While biomaterials provide a platform to control the delivery of vaccines, the recently discovered intrinsic inflammatory characteristics of many polymeric carriers can also complicate rational design because the carrier itself can alter the response to other vaccine components. To address this challenge, we recently developed immune-polyelectrolyte multilayer (iPEMs) capsules electrostatically assembled entirely from peptide antigen and molecular adjuvants. Here, we use iPEMs built from SIINFEKL model antigen and polyIC, a stimulatory toll-like receptor agonist, to investigate the impact of pH on iPEM assembly, the processing and interactions of each iPEM component with primary immune cells, and the role of these interactions during antigen-specific T cell responses in coculture and mice.

SUBMITTER: Chiu YC 

PROVIDER: S-EPMC4965838 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature