Calcium-dependent antigen binding as a novel modality for antibody recycling by endosomal antigen dissociation.

ABSTRACT: The pH-dependent antigen binding antibody, termed a recycling antibody, has recently been reported as an attractive type of second-generation engineered therapeutic antibody. A recycling antibody can dissociate antigen in the acidic endosome, and thus bind to its antigen multiple times. As a consequence, a recycling antibody can neutralize large amounts of antigen in plasma. Because this approach relies on histidine residues to achieve pH-dependent antigen binding, which could limit the epitopes that can be targeted and affect the rate of antigen dissociation in the endosome, we explored an alternative approach for generating recycling antibodies. Since calcium ion concentration is known to be lower in endosome than in plasma, we hypothesized that an antibody with antigen-binding propertie

SUBMITTER: Hironiwa N 

PROVIDER: S-EPMC4966519 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature