Use of magnetic hydrazide-modified polymer microspheres for enrichment of Francisella tularensis glycoproteins.

ABSTRACT: The field of microbial proteomics has currently experienced a boom in the discovery of glycosylated proteins of various pathogenic bacteria as potential mediators of host-pathogen interactions. The presence of glycoproteins has recently been discovered in a Gram-negative pathogenic bacterium Francisella tularensis, utilizing glycoprotein detection and isolation techniques in combination with mass spectrometry. The isolation of glycoproteins is a prerequisite for their subsequent mass-spectrometric identification. Current glycoprotein isolation/enrichment methods comprise lectin affinity chromatography, aminophenylboronic acid and hydrazide-based enrichment. The use of magnetic microspheres containing functional groups is nowadays among state-of-art separation methodologies owing to

SUBMITTER: Horak D 

PROVIDER: S-EPMC4966525 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature