Computational tool for the early screening of monoclonal antibodies for their viscosities.

ABSTRACT: Highly concentrated antibody solutions often exhibit high viscosities, which present a number of challenges for antibody-drug development, manufacturing and administration. The antibody sequence is a key determinant for high viscosity of highly concentrated solutions; therefore, a sequence- or structure-based tool that can identify highly viscous antibodies from their sequence would be effective in ensuring that only antibodies with low viscosity progress to the development phase. Here, we present a spatial charge map (SCM) tool that can accurately identify highly viscous antibodies from their sequence alone (using homology modeling to determine the 3-dimensional structures). The SCM tool has been extensively validated at 3 different organizations, and has proved successful in correctly id

SUBMITTER: Agrawal NJ 

PROVIDER: S-EPMC4966561 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature