Identification of crucial microRNAs and genes in hypoxia-induced human lung adenocarcinoma cells.

ABSTRACT:

Background

Variations of microRNA (miRNA) expression profile in hypoxic lung cancer cells have not been studied so far. Therefore, using miRNA microarray technology, this study aimed to study the miRNA expression profile and investigate the potential crucial miRNAs and their target genes in hypoxia-induced human lung adenocarcinoma cells.

Materials and methods

Based on miRNA microarray, miRNA expression profiling of hypoxia-induced lung adenocarcinoma A549 cells was obtained. After identification of differentially expressed miRNAs (DE-miRNAs) in hypoxic cells, target genes of DE-miRNAs were predicted, and functional enrichment analysis of targets was conducted. Furthermore, the expression levels of DE-miRNAs and their target genes were validated by real-time quantitative pol

SUBMITTER: Geng Y 

PROVIDER: S-EPMC4966693 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature