Seamless editing of the chloroplast genome in plants.

ABSTRACT:

Background

Gene editing technologies enable the precise insertion of favourable mutations and performance enhancing trait genes into chromosomes whilst excluding all excess DNA from modified genomes. The technology gives rise to a new class of biotech crops which is likely to have widespread applications in agriculture. Despite progress in the nucleus, the seamless insertions of point mutations and non-selectable foreign genes into the organelle genomes of crops have not been described. The chloroplast genome is an attractive target to improve photosynthesis and crop performance. Current chloroplast genome engineering technologies for introducing point mutations into native chloroplast genes leave DNA scars, such as the target sites for recombination enzymes. Seamless editing metho

SUBMITTER: Martin Avila E 

PROVIDER: S-EPMC4966725 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature