Cysteinylation of a monoclonal antibody leads to its inactivation.

ABSTRACT: Post-translational modifications can have a signification effect on antibody stability. A comprehensive approach is often required to best understand the underlying reasons the modification affects the antibody's potency or aggregation state. Monoclonal antibody 001 displayed significant variation in terms of potency, as defined by surface plasmon resonance testing (Biacore), from lot to lot independent of any observable aggregation or degradation, suggesting that a post-translational modification could be driving this variability. Analysis of different antibody lots using analytical hydrophobic interaction chromatography (HIC) uncovered multiple peaks of varying size. Electrospray ionization mass spectrometry (ESI-MS) indicated that the antibody contained a cysteinylation post-translation

SUBMITTER: McSherry T 

PROVIDER: S-EPMC4966827 | biostudies-literature | 2016 May-Jun

REPOSITORIES: biostudies-literature