Transpo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries.

ABSTRACT: In vitro antibody display and screening technologies geared toward the discovery and engineering of clinically applicable antibodies have evolved from screening artificial antibody formats, powered by microbial display technologies, to screening of natural, full-IgG molecules expressed in mammalian cells to readily yield lead antibodies with favorable properties in production and clinical applications. Here, we report the development and characterization of a novel, next-generation mammalian cell-based antibody display and screening platform called Transpo-mAb Display, offering straightforward and efficient generation of cellular libraries by using non-viral transposition technology to obtain stable antibody expression. Because Transpo-mAb Display uses DNA-transposable vectors with substan

SUBMITTER: Waldmeier L 

PROVIDER: S-EPMC4966844 | biostudies-literature | 2016 May-Jun

REPOSITORIES: biostudies-literature