Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity.

ABSTRACT: Basal bodies comprise nine symmetric triplet microtubules that anchor forces produced by the asymmetric beat pattern of motile cilia. The ciliopathy protein Poc1 stabilizes basal bodies through an unknown mechanism. In poc1∆ cells, electron tomography reveals subtle defects in the organization of intertriplet linkers (A-C linkers) that connect adjacent triplet microtubules. Complete triplet microtubules are lost preferentially near the posterior face of the basal body. Basal bodies that are missing triplets likely remain competent to assemble new basal bodies with nine triplet microtubules, suggesting that the mother basal body microtubule structure does not template the daughter. Our data indicate that Poc1 stabilizes basal body triplet microtubules through linkers between neighboring tri

SUBMITTER: Meehl JB 

PROVIDER: S-EPMC4966981 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature