Increased Sensitivity of HIV-1 p24 ELISA Using a Photochemical Signal Amplification System.

ABSTRACT:

Background

In this study we describe a photochemical signal amplification method (PSAM) for increasing of the sensitivity of enzyme-linked immunosorbent assay (ELISA) for determination of HIV-1 p24 antigen. The photochemical signal amplification method is based on an autocatalytic photochemical reaction of a horseradish peroxidase (HRP) substrate, orthophenylenediamine (OPD).

Methods

To compare the performance of PSAM-boosted ELISA with a conventional colorimetric ELISA for determination of HIV-1 p24 antigen we employed a PerkinElmer HIV-1 p24 ELISA kit, using conventional ELISA alongside ELISA + PSAM.

Results

In the present study, we show that PSAM technology allows one to increase the analytical sensitivity and dynamic range of a commercial HIV-1 p24 ELISA kit, with

SUBMITTER: Bystryak S 

PROVIDER: S-EPMC4967528 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature