Interplay of permanent charge and hydrophobicity in the electrospray ionization of glycans.

ABSTRACT: The analysis of N-linked glycans by mass spectrometry (MS) has been characterized by low signal-to-noise ratios and high limits of detection due to their hydrophilicity and lack of basic sites able to be protonated. As a result, every step in glycan sample preparation must be thoroughly optimized in order to minimize sample loss, contamination, and analytical variability. Importantly, properties of glycans and their derivatized counterparts must be thoroughly studied in order to exploit certain characteristics for enhancing MS analysis. Herein, the effectiveness of the incorporation of a permanent charge is studied and determined to hamper glycan analysis. Also, a procedure for glycan hydrazone formation is optimized and outlined where a large number of variables were simultaneously analyz

SUBMITTER: Walker SH 

PROVIDER: S-EPMC4967534 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature