Characterization of mAb dimers reveals predominant dimer forms common in therapeutic mAbs.

ABSTRACT: The formation of undesired high molecular weight species such as dimers is an important quality attribute for therapeutic monoclonal antibody formulations. Therefore, the thorough understanding of mAb dimerization and the detailed characterization mAb dimers is of great interest for future pharmaceutical development of therapeutic antibodies. In this work, we focused on the analyses of different mAb dimers regarding size, surface properties, chemical identity, overall structure and localization of possible dimerization sites. Dimer fractions of different mAbs were isolated to a satisfactory purity from bulk material and revealed 2 predominant overall structures, namely elongated and compact dimer forms. The elongated dimers displayed one dimerization site involving the tip of the Fab domai

SUBMITTER: Plath F 

PROVIDER: S-EPMC4968096 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature