Open data set of live cyanobacterial cells imaged using an X-ray laser.

ABSTRACT: Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-electron lasers circumvent this problem by outrunning key damage processes with an ultra-short and extremely bright coherent X-ray pulse. Diffraction-before-destruction experiments provide high-resolution data from cells that are alive when the femtosecond X-ray pulse traverses the sample. This paper presents two data sets from micron-sized cyanobacteria obtained at the Linac Coherent Light Source, containing a total of 199,000 diffraction patterns. Utilizing this type of diffraction data will require the development of new analysis methods and algorithms for studying structure and struc

SUBMITTER: van der Schot G 

PROVIDER: S-EPMC4968219 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature