Robust high-performance nanoliter-volume single-cell multiple displacement amplification on planar substrates.

ABSTRACT: The genomes of large numbers of single cells must be sequenced to further understanding of the biological significance of genomic heterogeneity in complex systems. Whole genome amplification (WGA) of single cells is generally the first step in such studies, but is prone to nonuniformity that can compromise genomic measurement accuracy. Despite recent advances, robust performance in high-throughput single-cell WGA remains elusive. Here, we introduce droplet multiple displacement amplification (MDA), a method that uses commercially available liquid dispensing to perform high-throughput single-cell MDA in nanoliter volumes. The performance of droplet MDA is characterized using a large dataset of 129 normal diploid cells, and is shown to exceed previously reported single-cell WGA methods in am

SUBMITTER: Leung K 

PROVIDER: S-EPMC4968760 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature