High HER2 protein levels correlate with increased survival in breast cancer patients treated with anti-HER2 therapy.

ABSTRACT:

Introduction

Current methods to determine HER2 (human epidermal growth factor receptor 2) status are affected by reproducibility issues and do not reliably predict benefit from anti-HER2 therapy. Quantitative measurement of HER2 may more accurately identify breast cancer (BC) patients who will respond to anti-HER2 treatments.

Methods

Using selected reaction monitoring mass spectrometry (SRM-MS), we quantified HER2 protein levels in formalin-fixed, paraffin-embedded (FFPE) tissue samples that had been classified as HER2 0, 1+, 2+ or 3+ by immunohistochemistry (IHC). Receiver operator curve (ROC) analysis was conducted to obtain optimal HER2 protein expression thresholds predictive of HER2 status (by standard IHC or in situ hybridization [ISH]) and of survival benefit after an

SUBMITTER: Nuciforo P 

PROVIDER: S-EPMC4968773 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature