Redundant roles of the phosphatidate phosphatase family in triacylglycerol synthesis in human adipocytes.

ABSTRACT:

Aims/hypothesis

In mammals, the evolutionary conserved family of Mg(2+)-dependent phosphatidate phosphatases (PAP1), involved in phospholipid and triacylglycerol synthesis, consists of lipin-1, lipin-2 and lipin-3. While mutations in the murine Lpin1 gene cause lipodystrophy and its knockdown in mouse 3T3-L1 cells impairs adipogenesis, deleterious mutations of human LPIN1 do not affect adipose tissue distribution. However, reduced LPIN1 and PAP1 activity has been described in participants with type 2 diabetes. We aimed to characterise the roles of all lipin family members in human adipose tissue and adipogenesis.

Methods

The expression of the lipin family was analysed in adipose tissue in a cross-sectional study. Moreover, the effects of lipin small interfering RNA (siRNA)-m

SUBMITTER: Temprano A 

PROVIDER: S-EPMC4969345 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature