Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.

ABSTRACT:

Aims/hypothesis

Per-Arnt-Sim kinase (PASK) is a nutrient-regulated domain-containing protein kinase previously implicated in the control of insulin gene expression and glucagon secretion. Here, we explore the roles of PASK in the control of islet hormone release, by generating mice with selective deletion of the Pask gene in pancreatic beta or alpha cells.

Methods

Floxed alleles of Pask were produced by homologous recombination and animals bred with mice bearing beta (Ins1 (Cre); PaskBKO) or alpha (Ppg (Cre) [also known as Gcg]; PaskAKO) cell-selective Cre recombinase alleles. Glucose homeostasis and hormone secretion in vivo and in vitro, gene expression and islet cell mass were measured using standard techniques.

Results

Ins1 (Cre)-based recombination led to efficie

SUBMITTER: Semplici F 

PROVIDER: S-EPMC4969360 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature