Pipeline for amplifying and analyzing amplicons of the V1-V3 region of the 16S rRNA gene.

ABSTRACT:

Background

Profiling of 16S rRNA gene sequences is an important tool for testing hypotheses in complex microbial communities, and analysis methods must be updated and validated as sequencing technologies advance. In host-associated bacterial communities, the V1-V3 region of the 16S rRNA gene is a valuable region to profile because it provides a useful level of taxonomic resolution; however, use of Illumina MiSeq data for experiments targeting this region needs validation.

Results

Using a MiSeq machine and the version 3 (300 × 2) chemistry, we sequenced the V1-V3 region of the 16S rRNA gene within a mock community. Nineteen bacteria and one archaeon comprised the mock community, and 12 replicate amplifications of the community were performed and sequenced. Sequencing the larg

SUBMITTER: Allen HK 

PROVIDER: S-EPMC4970291 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature