Dragonfly: an implementation of the expand-maximize-compress algorithm for single-particle imaging.

ABSTRACT: Single-particle imaging (SPI) with X-ray free-electron lasers has the potential to change fundamentally how biomacromolecules are imaged. The structure would be derived from millions of diffraction patterns, each from a different copy of the macromolecule before it is torn apart by radiation damage. The challenges posed by the resultant data stream are staggering: millions of incomplete, noisy and un-oriented patterns have to be computationally assembled into a three-dimensional intensity map and then phase reconstructed. In this paper, the Dragonfly software package is described, based on a parallel implementation of the expand-maximize-compress reconstruction algorithm that is well suited for this task. Auxiliary modules to simulate SPI data streams are also included to assess the

SUBMITTER: Ayyer K 

PROVIDER: S-EPMC4970497 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature