Longitudinal Metagenomic Analysis of Hospital Air Identifies Clinically Relevant Microbes.

ABSTRACT: We describe the sampling of sixty-three uncultured hospital air samples collected over a six-month period and analysis using shotgun metagenomic sequencing. Our primary goals were to determine the longitudinal metagenomic variability of this environment, identify and characterize genomes of potential pathogens and determine whether they are atypical to the hospital airborne metagenome. Air samples were collected from eight locations which included patient wards, the main lobby and outside. The resulting DNA libraries produced 972 million sequences representing 51 gigabases. Hierarchical clustering of samples by the most abundant 50 microbial orders generated three major nodes which primarily clustered by type of location. Because the indoor locations were longitudinally consistent, episodi

SUBMITTER: King P 

PROVIDER: S-EPMC4970769 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature