3D Imaging of Retinal Pigment Epithelial Cells in the Living Human Retina.

ABSTRACT:

Purpose

Dysfunction of the retinal pigment epithelium (RPE) underlies numerous retinal pathologies, but biomarkers sensitive to RPE change at the cellular level are limited. In this study, we used adaptive optics optical coherence tomography (AO-OCT) in conjunction with organelle motility as a novel contrast mechanism to visualize RPE cells and characterize their 3-dimensional (3D) reflectance profile.

Methods

Using the Indiana AO-OCT imaging system (λc = 790 nm), volumes were acquired in the macula of six normal subjects (25-61 years). Volumes were registered in 3D with subcellular accuracy, layers segmented, and RPE and photoreceptor en face images extracted and averaged. Voronoi and two-dimensional (2D) power spectra analyses were applied to the images to quantify RPE and

SUBMITTER: Liu Z 

PROVIDER: S-EPMC4970801 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature