Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest.

ABSTRACT: Many environmental and physiological stresses are chronic. Thus, cells are constantly exposed to diverse types of genotoxic insults that challenge genome stability, including those that induce oxidative DNA damage. However, most in vitro studies that model cellular response to oxidative stressors employ short exposures and/or acute stress models. In this study, we tested the hypothesis that chronic and repeated exposure to a micromolar concentration of hydrogen peroxide (H₂O₂) could activate DNA damage responses, resulting in cellular adaptations. For this purpose, we developed an in vitro model in which we incubated mouse myoblast cells with a steady concentration of ~50μM H₂O₂ for one hour daily for seven days, followed by a final challenge of

SUBMITTER: Santa-Gonzalez GA 

PROVIDER: S-EPMC4971155 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature