Excitation Light Dose Engineering to Reduce Photo-bleaching and Photo-toxicity.

ABSTRACT: It is important to determine the most effective method of delivering light onto a specimen for minimal light induced damage. Assays are presented to measure photo-bleaching of fluorophores and photo-toxicity to living cells under different illumination conditions. Turning the light off during part of the experimental time reduced photo-bleaching in a manner proportional to the time of light exposure. The rate of photo-bleaching of EGFP was reduced by 9-fold with light pulsing on the micro-second scale. Similarly, in living cells, rapid line scanning resulted in reduced cell stress as measured by mitochondrial potential, rapid cell protrusion and reduced cell retraction. This was achieved on a commercial confocal laser scanning microscope, without any compromise in image quality, by using r

SUBMITTER: Boudreau C 

PROVIDER: S-EPMC4971503 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature