Selective AKT Inhibition by MK-2206 Represses Colorectal Cancer-Initiating Stem Cells.

ABSTRACT:

Background

Colorectal cancer (CRC) is a leading cause of cancer-related death worldwide. Growing evidence indicates that tumor-initiating cells (TICs) are responsible for tumor growth and progression. Conventional chemotherapeutics do not sufficiently eliminate TICs, leading to tumor relapse. We aimed to gain insight into TIC biology by comparing the transcriptome of primary TIC cultures and their normal stem cell counterparts to uncover expression differences.

Methods

We established colonosphere cultures derived from the resection of paired specimens of primary tumor and normal mucosa in patients with CRC. These colonospheres, enriched for TICs, were used for differential transcriptome analyses to detect new targets for a TIC-directed therapy. Effects of target inhibition o

SUBMITTER: Malkomes P 

PROVIDER: S-EPMC4972858 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature