Autofluorescence multiphoton microscopy for visualization of tissue morphology and cellular dynamics in murine and human airways.

ABSTRACT: The basic understanding of inflammatory airway diseases greatly benefits from imaging the cellular dynamics of immune cells. Current imaging approaches focus on labeling specific cells to follow their dynamics but fail to visualize the surrounding tissue. To overcome this problem, we evaluated autofluorescence multiphoton microscopy for following the motion and interaction of cells in the airways in the context of tissue morphology. Freshly isolated murine tracheae from healthy mice and mice with experimental allergic airway inflammation were examined by autofluorescence multiphoton microscopy. In addition, fluorescently labeled ovalbumin and fluorophore-labeled antibodies were applied to visualize antigen uptake and to identify specific cell populations, respectively. The trachea in livin

SUBMITTER: Kretschmer S 

PROVIDER: S-EPMC4972900 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature