ABSSeq: a new RNA-Seq analysis method based on modelling absolute expression differences.

ABSTRACT:

Background

The recent advances in next generation sequencing technology have made the sequencing of RNA (i.e., RNA-Seq) an extemely popular approach for gene expression analysis. Identification of significant differential expression represents a crucial initial step in these analyses, on which most subsequent inferences of biological functions are built. Yet, for identification of these subsequently analysed genes, most studies use an additional minimal threshold of differential expression that is not captured by the applied statistical procedures.

Results

Here we introduce a new analysis approach, ABSSeq, which uses a negative binomal distribution to model absolute expression differences between conditions, taking into account variations across genes and samples as well as

SUBMITTER: Yang W 

PROVIDER: S-EPMC4973090 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature